PCR-Based DNA Detection of Bovine on Medium of Cultured Meat for Consumption: Religious Perspectives

Authors

  • MOHD IZHAR ARIFF MOHD KASHIM Research Centre of Sharia, Faculty of Islamic Studies, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Institute of Islam Hadhari, University Kebangsaan Malaysia, 43600, Bangi, Selangor
  • NUR ASMADAYANA HASIM Pusat Pengajian Citra Universiti, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor
  • KIPLI YASSIN Institute of Islam Hadhari, Universiti Kebangsaan, Malaysia,43600, Bangi, Selangor, Jabatan Mufti Sarawak, 8, Lorong P. Ramlee 5, KTLD, 93400 Kuching, Sarawak
  • ALIA ARYSSA ABDUL HARIS Department of Food Science, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor
  • FARAH AYUNI MOHD HATTA Institute of Islam Hadhari, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor
  • NURUL HAFIZAH MOHD NOR Institute of Islam Hadhari, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor
  • LENNY SURYANI SAFRI Department of Food Science, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor
  • KAMARULZAMAN MUSTAPPA Research Centre of Sharia, Faculty of Islamic Studies, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor
  • NOOR LIZZA MOHAMED SAID Research Centre of Sharia, Faculty of Islamic Studies, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor
  • DIANI MARDIANA MAT ZIN Kolej Permata Insan, Universiti Sains Islam Malaysia, 71800, Nilai, Negeri Sembilan

DOI:

https://doi.org/10.61707/qx6f8x51

Keywords:

Cultured Meat, Polymerase Chain Reaction, Cultured Medium, Halal, Kosher

Abstract

Food technology advancements have led to the development of cultured or artificial meat produced in a laboratory setting. Recently, Israel and Singapore have built factories specifically for cultured meat production. Malaysia is also informed about a cultured meat processing facility, which is expected to be completed in 2024.  This alternative is a viable solution to meet the boosting needs and demand for meat-based food products while minimising the environmental impact of traditional livestock farming. Nevertheless, despite its potential benefits, cultured meat faces diverse challenges, including religious concerns for faiths with specific dietary requirements. Therefore, this research intended to determine the detection of DNA of bovine on the cultured medium by using polymerase chain reaction (PCR) analysis. The polymerase chain reaction analysis was conducted by targeting the mitochondrial DNA of the cytochrome oxidase II (COII) gene sequence and produced an amplicon size of 165 bp. The PCR was obtained by using the sample of medium mix with different concentrations (10-20%) of Foetal Bovine Serum (FBS) (Capricorn Scientific, Ebsdorfergrund, Germany), and the cell was harvested on different days. For DNA extraction, GENEAID Blood/Cell DNA mini kit (Taipei, Taiwan) was used. The findings demonstrate that DNA concentration in foetal bovine serum content was detected on the cultured medium. The presence of DNA contradicts religions such as Islam and Judaism that have strict standard dietary practices known as halal and kosher respectively. This study serves as a reference for the consumption of cultured meat for the consumer, particularly for Muslim and Jewish communities. 

Downloads

Published

2024-06-23

Issue

Section

Articles

How to Cite

PCR-Based DNA Detection of Bovine on Medium of Cultured Meat for Consumption: Religious Perspectives. (2024). International Journal of Religion, 5(10), 1917-1926. https://doi.org/10.61707/qx6f8x51

Similar Articles

1-10 of 119

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)